To be held in conjunction with the Joint Meeting of Ichthyologists and Herpetologists
- 1 Workshop goals
- 2 Program
- 3 Sponsors and organizers
- 4 Abstracts
- 4.1 A gentle introduction to ontologies for biology
- 4.2 Linking animal models and human diseases
- 4.3 Phenoscape: Using ontologies to link comparative morphology to genes
- 4.4 Why ontologies are important for understanding morphological images
- 4.5 An ontology for Xenopus anatomy and development
- 4.6 Development of an anatomical ontology for amphibians
- 4.7 Bgee: Integrating ontology and homology for the study of gene expression evolution
Ontologies, controlled vocabularies with well-defined relations among terms, are a key tool in scientific data integration. By using ontologies, scientists from different disciplines can know when they are referring to the same entity by different names, and new discoveries are enabled by computer software being able to reason across disciplines and over large datasets. Already widely used in genomics, ontologies are of growing importance in systematics, ecology, behavior, development, morphology and physiology. This workshop aims to explore the utility of ontologies for ichthyology and herpetology, using the Teleost Anatomy Ontology and the Amphibian Anatomy Ontology as case studies of community resources that are being actively developed and used by members of ASIH. Participants will present examples of how these ontologies are being used to provide new ways of exploring data within morphological and phenotypic databases. Talks in the morning will be followed in the afternoon with ontology development workshops and hands-on demonstrations of Phenoscape and AmphibAnat tools.
TIME: Saturday, July 25, 2009
PLACE: Hilton Portland & Executive Tower
- 8.00-8.30 Hilmar Lapp, National Evolutionary Synthesis Center: A gentle introduction to ontologies for biology (Abstract | Presentation)
- 8:30-9:00 Monte Westerfield, Director, Zebrafish Information Network and Institute of Neuroscience, Eugene, OR: Linking animal models and human diseases (Abstract | Presentation)
- 9:00-9:30 Paula Mabee, University of South Dakota: Phenoscape: Using ontologies to link comparative morphology to genes (Abstract | Presentation)
- 9:30—10:00 Greg Riccardi, Florida State University: Why ontologies are important for understanding morphological images (Abstract | Presentation)
- 10:00-10:30 Coffee
- 10:30-11:00 Peter Vize, University of Calgary: The Xenopus ontology and database (Abstract | Presentation)
- 11:00-11:30 Anne Maglia, Missouri University of Science and Technology: Development of an anatomical ontology for amphibians (Abstract | Presentation)
- 11:30—12:00 Marc Robinson-Rechavi, Universite de Lausanne, Switzerland: Integrating ontology and homology for the study of gene expression evolution (Abstract | Presentation)
- 1:30-4:30 Ontology development workshop and hands-on demonstrations of Phenoscape and AmphibAnat tools
Sponsors and organizers
The workshop is jointly sponsored by the Phenoscape and AmphibAnat projects, and is being held in conjunction with ASIH. The organizers include:
- Paula Mabee, Professor, Dept. of Biology, University of South Dakota
- Anne Maglia, Professor, Missouri University of Science and Technology
- Todd Vision, Assistant Director, National Evolutionary Synthesis Center (NESCent) and Associate Professor, Dept. of Biology, University of North Carolina
- Monte Westerfield, Director, Zebrafish Information Network and Professor, Institute of Neuroscience, University of Oregon
A gentle introduction to ontologies for biology
Hilmar Lapp (1), Todd J. Vision (1,2)
- US National Evolutionary Synthesis Center
- University of North Carolina at Chapel Hill
As biology has become increasingly data-rich, the reliance on databases and computation to integrate and mine the vast body of knowledge traditionally reported in the literature has grown dramatically. This has inspired the development of computational technologies to allow the exploration and linking of diverse types of data across biological databases at unprecedented scales. One of the most important technologies is an “ontology”, a hierarchically structured, controlled vocabulary of well-defined terms and the logical relationships that hold between them. Ontologies have been applied with tremendous success to transform scientific results traditionally reported as free text into a digital representation that is unambiguous, uniform across disciplines, and readily computable. For example, ontologies of biochemical functions and biological processes are used to unambiguously record what is known about a gene's function. Anatomy ontologies are now being used to unequivocally describe the morphological characteristics visible in a specimen image. Efforts (including two in ichthyology and herpetology) are underway to develop ontologies that will ultimately span the breadth of biological knowledge, which will have a profound impact on the way that biologists interact with data collections in the future. In order to be useful community resources, ontologies must accurately capture the state of biological knowledge, which requires that the biological community through their experts play an active role in their development. Here, I will provide a beginner’s guide to the world of ontologies and offer a roadmap for how biologists can best take advantage of, and contribute to, the growing suite of bio-ontology resources.
Linking animal models and human diseases
Director, Zebrafish Information Network and Institute of Neuroscience, University of Oregon
Phenotypes are the result of interactions of the whole genome with the environment. Studies that correlate genotype with phenotype are crucial for unraveling biological pathways and gene product interactions and, hence, are required for reaching the long-term goal of understanding how genes regulate developmental and physiological processes. Together with the NCBO and FlyBase we developed a bipartite “EQ” (Entity + Quality) syntax to describe phenotypes. The Entity is the part of the phenotype being described, the Quality describes how the entity is affected. The entities may be terms from anatomical ontologies or the Gene Ontology (GO; for biological processes, cellular components, and molecular functions). The Quality terms come from the Phenotype and Trait Ontology (PATO) that provides a hierarchy of qualitative or quantitative qualities that may be applied to an observable structure or process. We used EQ syntax and ontologies to annotate human disease genes (OMIM), and their Drosophila and Zebrafish homologs. We show that these data can be comparatively queried by phenotype alone, using an information content-based similarity search algorithm. To test whether usage of EQ syntax and the PATO ontology is sufficiently reproducible for annotating phenotypes, three curators independently annotated the same records. Differences in the annotations recorded by the three curators may arise from deficiencies in PATO, the anatomy or Gene ontologies, or the syntax itself. A comparison of these annotations allows testing and development of curatorial standards for phenotype annotation.
Phenoscape: Using ontologies to link comparative morphology to genes
University of South Dakota
Decades of comparative anatomical studies in ichthyology and herpetology have resulted in a rich body of ‘free-text’ data. As these data grow, they are increasingly hard to align and synthesize across taxonomic groups, and synthetic questions concerning the developmental and genetic basis of evolutionary changes in morphology cannot be easily or efficiently addressed. In order for this volume of comparative anatomical data to be analyzed in a developmental genetic context, it must first be rendered computable. One way to achieve this is to use ontologies. Using ostariophysan fishes as a prototype, the Phenoscape project has developed a system that includes ontologies representing expert knowledge of anatomy and taxonomy (the Teleost Anatomy Ontology and the Teleost Taxonomy Ontology), software for data curation (Phenex), and a knowledgebase that supports ontology-based reasoning about evolutionary phenotype data (PhenoscapeKB, http://phenoscape.org/kb). To date, over 5,000 characters from the phylogenetic literature have been annotated for 8,300 species, resulting in over eight million annotated phenotypes. PhenoscapeKB combines these evolutionary phenotypes with information about genetically characterized phenotype from ZFIN, the zebrafish community database. Through ontology-based reasoning over expert knowledge in taxonomy, comparative anatomy and developmental genetics, PhenoscapeKB can be used to address a host of questions spanning the domains of genetics, development and evolutionary biology, such as the nature of the genetic changes underlying phenotypic variation among taxa in nature.
Why ontologies are important for understanding morphological images
Greg Riccardi (1) and Austin Mast (2)
- College of Information, Florida State University
- Department of Biological Science, Florida State University, firstname.lastname@example.org
This talk will discuss some ways that image management and annotation, ontology development, and studies of physical features of organisms are integrated to facilitate research in evolution and development. The management of metadata and annotations of images is a primary capability of the Morphbank system, an on-line image repository system that has over 250,000 images of a variety of organisms. The metadata in Morphbank for an image includes information about the content of the image—that is, characteristics of the objects shown in the image. This includes information about the specimen with Darwin Core fields and information about the anatomy and views that are presented. Morphbank and Phenoscape have combined to integrate anatomical and phenotype annotations on images. An image of a portion of a fish skeleton in Morphbank includes references to the Teleost ontology terms that describe that bone (c. f., http://www.morphbank.net?id=459110). 1500 skeletal images from CToL are currently in Morphbank. A Search of Morphbank for the term will produce a collection of relevant images. An annotation of an image in Morphbank attaches one or more ontology terms to a specific part of the image. The integration of Morphbank with the Morphster ontology browser [ref] provides an illustration of ontology terms. A user may select a term in Morphster and see annotated Morphbank images that are relevant to that term. The inference capabilities of Morphster allow a user to find all images of “bone” or just those of “ceratobranchial” or those that exhibit a specific phenotype.
An ontology for Xenopus anatomy and development
Peter Vize, Erik Segerdell and Jeff Bowes
University of Calgary, Calgary, Alberta, Canada
The frogs Xenopus laevis and Xenopus (Silurana) tropicalis are model systems that have produced a wealth of genetic, genomic, and developmental information. Xenbase is a model organism database that provides centralized access to this information, including gene function data from high-throughput screens and the scientific literature. A controlled, structured vocabulary for Xenopus anatomy and development is essential for organizing these data. We have constructed a Xenopus anatomical ontology that represents the lineage of tissues and the timing of their development. We have classified many anatomical features in a common framework that has been adopted by several model organism database communities. The ontology is available for download at the Open Biomedical Ontologies Foundry. The Xenopus Anatomical Ontology will be used to annotate Xenopus gene expression patterns and mutant and morphant phenotypes. Its robust developmental map will enable powerful database searches and data analyses.
Development of an anatomical ontology for amphibians
Anne Maglia (1), Jennifer Leopold (1), Susan Gauch (2), Analia Pugener (1)
- Missouri University of Science and Technology, Rolla, MO, United States
- University of Arkansas, Fayetteville, AR, United States
Herein, we describe our ongoing efforts to develop a robust ontology for amphibian anatomy (www.amphibanat.org) that accommodates the diversity of anatomical structures present in the group. We discuss the design and implementation of the project, current resolutions to issues we have encountered, and future enhancements to the ontology. We also comment on efforts to integrate other data sets with the amphibian anatomical ontology.
Bgee: Integrating ontology and homology for the study of gene expression evolution
Department of Ecology and Evolution, Biophore, Lausanne University and Swiss Institute of Bioinformatics
The study of the evolution of developmental processes (evo-devo) has shown that the primary source of change in the evolution of phenotypes is changes in gene expression. Comparing gene expression patterns between animals thus is a major step to understand their evolution. This approach requires dedicated bioinformatics tools to perform high throughput analyses. Thus we have developed Bgee, a database designed to compare expression patterns between animals, by implementing ontologies describing anatomies and developmental stages of species, and then designing homology relationships between anatomies and comparison criteria between developmental stages. To define homology relationships between anatomical features we have developed the software Homolonto, which uses a modified ontology alignment approach to propose homology relationships between ontologies. Bgee then uses these aligned ontologies, onto which heterogeneous expression data types are mapped. These include microarrays, in situ hybridization, and ESTs, from human, mouse, xenopus and zebrafish. Bgee is available at http://bgee.unil.ch/