The Phenoscape Project uses community anatomy, quality, spatial, and taxonomic ontologies for the annotation of phenotypes from the comparative biology literature. The development of these ontologies follows OBO Foundry principles as much as possible, including reuse of ontology terms as the preferred mechanism for using shared terms. These ontologies can be downloaded, searched, and visualized at Ontobee, NCBO BioPortal, or downloaded from OBO Foundry. Ontologies can also be edited and viewed using the Protégé desktop software.
We use and contribute to the Uberon Anatomy Ontology, into which the Teleost Anatomy Ontology, Amphibian Anatomy Ontology, and Vertebrate Skeletal Anatomy Ontology were merged. More information about the development of Uberon can be found in Mungall et al. (2012) and Haendel et al. (2014).
Uberon is separated into a core file and an “extension” file (phenoscape-ext) which allows for separate editing by Phenoscape curators and the core Uberon developers. New terms required by Phensocape for annotation of vertebrate phenotypes are added to phenoscape-ext, which also contains the terms sourced from the TAO, AAO, and VSAO. We use the Protégé desktop software to edit phenoscape-ext. The release version of Uberon contains both core and ext terms in a single file.
phenoscape-ext.owl is the editors version. The release uberon/ext.owl retains the import chains and is pre-reasoned. uberon/ext.obo merges the import chain into one ontology because obo does not have good support for imports. Many of the other uberon derived artifacts such as composite-metazoan do not use ext but this may be changed in the future. See [http://uberon.org; uberon.org] for details of all the various cuts.
VSAO contains terms representing structures in the skeletal system of vertebrates. It references terms from the Common Anatomy Reference Ontology (CARO), Gene Ontology (GO) Biological Process, Cell Ontology (CL), and the Phenotype and Trait Ontology (PATO). VSAO can be downloaded at the OBO Foundry and browsed at BioPortal. Development of the VSAO is described in Dahdul et al. (2012).
The VSAO as released represents the outcome of the skeletal anatomy workshop held at NESCent and corresponding paper in PLOS ONE. New skeletal terms for the limb/fin and cranial skeleton have and will continue to be added to the Phenoscape-ext ontology based on work done at the Phenotype RCN Vertebrate Working Group meeting in Boulder, CO (June 1-3, 2011) and from ongoing work.
TAO is a multi-species ontology for teleost fishes that was initialized with terms from the Zebrafish Anatomical Ontology (ZFA). The development of the TAO focused on the skeletal system because it varies significantly across fishes, is well-preserved in fossil specimens, and it is often the focus of morphologically-based evolutionary studies in ichthyology. The development of the TAO is described in Dahdul et al. (2010). TAO can be downloaded from the OBO Foundry and browse at BioPortal. Further documentation about TAO is described here.
AAO is a multispecies ontology for amphibian anatomy. Development of AAO is described in Maglia et al. (2007). View AAO at the BioPortal. The last updated version (April 2012) includes terms merged from the Xenopus Anatomy Ontology and updates from David Blackburn and Wasila Dahdul.
Phenotype data for model organisms are imported into the Phenoscape KB for mouse (MGI), human (HPO), zebrafish (ZFIN), and frog (Xenbase). Uberon maintains cross-references to terms from the corresponding model organism anatomy ontologies.
The Adult Mouse Anatomy (MA) ontology contains terms that represent structures in the postnatal mouse (Mus) and is used to annotate gene expression and phenotypes for the mouse at Mouse Genome Informatics (MGI) and other resources. Development of the MA is described in Hayamizu et al. (2005).
XAO contains terms that represent anatomical structures for the model organism Xenopus laevis (African clawed frog). Xenbase uses XAO to annotate Xenopus gene expression and phenotypes.
ZFA is used by ZFIN to annotate mutant phenotypes for the model organsim zebrafish (Danio rerio).
ZFA terms are cross referenced to TAO terms, and these cross references will be maintained and updated as needed.
Previously, Phenoscape coordinated the development and integration of vertebrate skeletal terms across anatomy ontologies for vertebrates, with taxonomic focus on teleosts, zebrafish, amphibians, Xenopus, amniotes, and mouse. The limb/fin branch was the primary focus of ontology development in Phenoscape II. We held regular project anatomy ontology conference calls to discuss ontology development with the curators of the various anatomy ontologies (ZFA, XAO, MA). We also coordinated our development calls with the Phenotype RCN vertebrate working group. Currently, we use and contribute new terms to the Uberon anatomy ontology (metazoans) into which the Teleost Anatomy Ontology, Amphibian Anatomy Ontology, and Vertebrate Skeletal Anatomy Ontology were merged.
The Vertebrate Taxonomy Ontology (Midford, Dececchi, et al. 2013) includes the TTO, ATO, and uses the NCBI taxonomy as a source covering amniotes and basal chordates outside the scope of the TTO. The ontology has an assigned OBO purl http://purl.obolibrary.org, and there is a repository at https://code.google.com/p/vertebrate-taxonomy-ontology/. The current release covers vertebrates and was built by starting with the NCBI taxonomy for vertebrates and splicing in TTO (except hagfish), and the downloadable amphibiaweb taxonomy. Subspecies names were added to their parent species as synonyms (not subclasses). The taxonomy is currently in the available in OWL format, as generated by the OBO release tool. It also uses the Taxonomic Rank Vocabulary to tag taxa with specified rank.
These can provide links to additional synonyms and resources (e.g., TTO uses fishbase to provide common names and links to their pages). Taxonomic synonyms are particularly useful as aids to data curation, but common names can assist users in browsing the website. Taxonomic resources (above) can be used as sources of names as well.
The Teleost Taxonomy Ontology (TTO) is derived from the Catalog of Fishes (see also the representation on BioPortal, which can be navigated on-line). The TTO was developed from the Phenoscape I project and provides coverage of fish in the VTO. The TTO is updated in concert with Catalog of Fishes updates.
During 2010, we released a separate Taxonomic Rank Vocabulary, and removed all rank terms (e.g., family, genus, etc.) from the taxa within the TTO. Taxa in the TTO specify their ranks as property values via the metadata relation has_rank, but the object of the has_rank links is contained in the TAXRANK vocabulary.
Developing TAXRANK as a vocabulary, rather than an ontology (e.g., by defining an ordering relation between ranks) should facilitate its reuse in other taxonomic ontologies. Developing a cross-authority (e.g., ICZN, ICBN, etc.) ontology of ranks may be possible, but there does not appear to be a compelling need for such an ontology.
There is a flat vocabulary of fish collections, based on a list used in Catalog of Fishes, though with a few additions listed on the Fish Collection Updates page. The master list, from which the OBO-format ontology is generated is available as a google docs spreadsheet. It has been augmented with links to entries in the Biodiversity Collections Index.
The current release, as used in Phenex is Fish Collection Abbreviations
The ATO has been deprecated in favor of generating the Amphibian component of the VTO directly from Amphibiaweb.
These ontologies listed below were initiated by the model organism communities. Phenoscape is actively involved in extending these ontologies.